Product: Anti-ICE Protease Activating Factor pAb
Cat. No.: PC-457 (100 μg)
Synonyms:
Ipaf, CARD12, CLANA.
Description:
Apoptosis is related to many diseases and
induced by a family of cell death receptors and
their ligands. Cell death signals are transduced
by death domain containing adaptor molecules
and proteases including several members of the
caspase family. Another family of proteins that
functions as a critical regulator of apoptosis and
NF-kB signaling pathways is the CED-4/Apaf-1
(apoptosis protein activating factor-1) protein
family. Ipaf (ICE protease activating factor) is a
CED-4/Apaf-1 family member that activates
caspase-1/ICE and can induce apoptosis in
human cells in a caspase-1 dependent manner.
Ipaf and caspase-1 are thought to interact with
each other through the association of the Ipaf
amino-terminal CARD (caspase recruitment
domain) and amino-terminal CARD of caspase-
1.
Host:
Rabbit
Species Reactivity:
Human
Positive Control:
Human peripheral blood leukocyte (PBL) cell
lysate. A 110 kDa band can be detected.
Immunogen:
A synthetic peptide corresponding to amino
acids near the C-terminus of human Ipaf
(Genbank accession NP_067032).
Format:
100 μg at 1 mg/mL of immunaffinity-purified IgG
in PBS containing 0.02 sodium azide.
Storage:
Store at 4°C.
Applications:

Western blot: Use at 0.5–2.0 μg/mL.

Immunohistochemistry: Use at about 10
μg/mL inTHP-1 cells.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product.