Product: Anti-IKKa (C2), Polyclonal
Cat. No: PC-041 (100 μg)
Background:
Nuclear factor kappa b (NF-kb) is a ubiquitous
transcription factor and an essential mediator of
gene expression during activation of immune
and inflammatory responses. NF-kb mediates
the expression of a great variety of genes in
response to extracellular stimuli including IL-1,
TNFa and bacteria product LPS. NF-kb is
associated with Ikb proteins in the cell cytoplasm,
which inhibit NF-kb activity. The long-sought Ikb
kinase (IKK), which phosphorylates Ikb, and
mediates Ikb degradation and NF-kB activation,
was recently identified by several laboratories.
IKK is a serine protein kinase, and the IKK
complex contains alpha and beta subunits (IKKa
and IKKb). IKKa and IKKb interact with each
other and both are essential for the NF-kb
activation. IKKa specifically phosphorylates Ikb-
a. IKKa is expressed in variety of human tissues.
Specificity:
Binds IKKa. It has no cross response to IKKb or
IKKg
Positive Control: Whole cell lysate from HeLa or
Jurkat cells. An 85 kDa band should be
detected by Western blot.
Species Reactivity:
Human, other species not tested.
Ig Isotype:
Rabbit IgG
Immunogen:
A peptide corresponding to amino acids 699 to
715 of human IKKa, which differs from
corresponding murine sequence by four amino
acids.
Format:
Immunoaffinity chromatography purified IgG,
100 μg in 200 μl of PBS containing 0.02
sodium azide.
Storage and Stability:
Stable for at least one year when stored at +4°C.
Applications and Suggested Dilutions:

Western blot: Suggest 1:500 to 1:1000.
The optimal dilution for a specific application
should be determined by the researcher.
Limitations:
For in vitro research use only. Not for use in
diagnostics or in humans.
Warranty:
No warranties, expressed or implied, are made
regarding the use of this product. KAMIYA
BIOMEDICAL COMPANY is not liable for any
damage, personal injury, or economic loss
caused by this product