Human Stem Cell Factor Retroviral Vector Set (6 Genes)
产品名称： Human Stem Cell Factor Retroviral Vector Set (6 Genes)
英文名称： Human Stem Cell Factor Retroviral Vector Set (6 Genes)
产品编号： RTV-709-C
产品价格： null
产品产地： USA
品牌商标： cellbiolabs
更新时间： 2023-08-17T13:40:59
使用范围：
翌圣生物科技（上海）股份有限公司
- 联系人 : 李自转
- 地址 : 上海市浦东新区天雄路166弄一号楼三层南单元
- 邮编 : 200030
- 所在区域 : 上海
- 电话 : 139****5640
- 传真 : 021-34615995-188
- 邮箱 : lizizhuan@yeasen.com
Kit Components 1. pMXs-hOct3/4 (Part No 370101): One vial containing 10 μg at 0.25 μg/μL in TE. 2. pMXs-hSox2 (Part No 370102): One vial containing 10 μg at 0.25 μg/μL in TE. 3. pMXs-hc-Myc (Part No 370103): One vial containing 10 μg at 0.25 μg/μL in TE. 4. pMXs-hKlf4 (Part No 370104): One vial containing 10 μg at 0.25 μg/μL in TE. 5. pMXs-hNANOG (Part No 370901): One vial containing 10 μg at 0.25 μg/μL in TE. 6. pMXs-hLim-28 (Part No 370902): One vial containing 10 μg at 0.25 μg/μL in TE.
Background
Retroviruses are efficient tools for delivering heritable genes into the genome of dividing cells. Cell
Biolabs’ pMXs retroviral vector is based on Moloney murine leukemia virus (MMLV). The vector
provides the viral package signal, transcription and processing elements, and MCS for cloning of a target
gene. The viral env gene, produced by the package cell line, encodes the envelop protein, which
determines the viral infectivity range. Transfection into a package cell line produces high-titer,
replication-incompetent viruses.
Induced pluripotent stem (iPS) cells can be generated from various somatic cells by the retrovirusmediated
transfection of four transcription factors, namely Oct3/4, Sox2, c-Myc, and Klf4 (Yamanaka’s
laboratory) or Oct3/4, Sox2, NANOG, and Lin-28 (Thomson’s laboratory). It has been shown that a
combination of all 6 transcription factors (Oct3/4, Sox2, c-Myc, Klf4, NANOG and Lin-28), significantly
increases the efficiency of generating iPS cells from somatic cells. iPS cells are indistinguishable from
ES cells in morphology, proliferation, gene expression, and teratoma formation. Furthermore, when
transplanted into blastocysts, iPS cells can give rise to adult chimeras, which are competent for germline
transmission.