BRMS1 monoclonal antibody (M01), clone 2D4-2G11
产品名称： BRMS1 monoclonal antibody (M01), clone 2D4-2G11
英文名称： BRMS1 monoclonal antibody (M01), clone 2D4-2G11
产品编号： H00025855-M01
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a full length recombinant BRMS1.
- Immunogen:
- BRMS1 (AAH09834, 1 a.a. ~ 246 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- MPVQPPSKDTEEMEAEGDSAAEMNGEEEESEEERSGSQTESEEESSEMDDEDYERRRSECVSEMLDLEKQFSELKEKLFRERLSQLRLRLEEVGAERAPEYTEPLGGLQRSLKIRIQVAGIYKGFCLDVIRNKYECELQGAKQHLESEKLLLYDTLQGELQERIQRLEEDRQSLDLSSEWWDDKLHARGSSRSWDSLPPSKRKKAPLVSGPYIVYMLQEIDILEDWTAIKKARAAVSPQKRKSDGP
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG1 Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (52.8 KDa) .
- MSDS:
Download
- Publication Reference
- 1.
- Low BRMS1 expression promotes nasopharyngeal carcinoma metastasis in vitro and in vivo and is associated with poor patient survival.
Cui RX, Liu N, He QM, Li WF, Huang BJ, Sun Y, Tang LL, Chen M, Jiang N, Chen L, Yun JP, Zeng J, Guo Y, Wang HY, Ma J.BMC Cancer. 2012 Aug 29;12(1):376.
- 2.
- Breast Cancer Metastasis Suppressor 1 (BRMS1) is destabilized by the Cul3-SPOP E3 ubiquitin ligase complex.
Kim B, Nam HJ, Pyo KE, Jang MJ, Kim IS, Kim D, Boo K, Lee SH, Yoon JB, Baek SH, Kim JH.Biochem Biophys Res Commun. 2011 Nov 9.
- 3.
- BRMS1 transcriptional repression correlates with CpG island methylation and advanced
pathologic stage in non-small cell lung cancer.
Nagji AS, Liu Y, Stelow EB, Stukenborg GJ, Jones DR.The Journal of Pathology (2010), DOI: 10.1002/path.2707
- 4.
- Breast cancer metastasis suppressor 1 (BRMS1) suppresses metastasis and correlates with improved patient survival in non-small cell lung cancer.
Smith PW, Liu Y, Siefert SA, Moskaluk CA, Petroni GR, Jones DR.Cancer Lett. 2009 Apr 18;276(2):196-203. Epub 2008 Dec 25.
- 5.
- Proteomics-Based Strategy To Delineate the Molecular Mechanisms of the Metastasis Suppressor Gene BRMS1.
Rivera J, Megias D, Bravo J.J Proteome Res. 2007 Oct;6(10):4006-18. Epub 2007 Sep 14.
- 6.
- Breast cancer metastasis suppressor 1 functions as a corepressor by enhancing HDAC1-mediated deacetylation of RelA/p65 and promoting apoptosis.
Liu Y, Smith PW, Jones DR.Mol Cell Biol. 2006 Dec;26(23):8683-96. Epub 2006 Sep 25.
- Applications
- Western Blot (Tissue lysate)
- BRMS1 monoclonal antibody (M01), clone 2D4-2G11. Western Blot analysis of BRMS1 expression in human liver.
- Protocol Download
- Western Blot (Transfected lysate)
- Western Blot analysis of BRMS1 expression in transfected 293T cell line by BRMS1 monoclonal antibody (M01), clone 2D4-2G11.
Lane 1: BRMS1 transfected lysate (Predicted MW: 28.5 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunoprecipitation
- Immunoprecipitation of BRMS1 transfected lysate using anti-BRMS1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with BRMS1 MaxPab rabbit polyclonal antibody.
- Protocol Download
- Sandwich ELISA (Recombinant protein)
- Detection limit for recombinant GST tagged BRMS1 is approximately 3ng/ml as a capture antibody.
- Protocol Download
- Entrez GeneID:
- 25855
- GeneBank Accession#:
- BC009834
- Protein Accession#:
- AAH09834
- Gene Name:
- BRMS1
- Gene Alias:
- DKFZp564A063
- Gene Description:
- breast cancer metastasis suppressor 1
- Omim ID:
- 606259
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene reduces the metastatic potential, but not the tumorogenicity, of human breast cancer and melanoma cell lines. The protein encoded by this gene localizes primarily to the nucleus and is a component of the mSin3a family of histone deacetylase complexes (HDAC). The protein contains two coiled-coil motifs and several imperfect leucine zipper motifs. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq
- Other Designations:
- breast cancer metastasis-suppressor 1