Introduction
Free radicals and other reactive species are constantly generated in vivo and cause oxidative damage to biomolecules, a process held in check only by the existence of multiple antioxidant and repair systems as well as the replacement of damaged nucleic acids, proteins and lipids. DNA is probably the most biologically significant target of oxidative attack, and it is widely thought that continuous oxidative damage to DNA is a significant contributor to the age-related development of the major cancers, such as those of the colon, breast, rectum, and prostate. Among numerous types of oxidative DNA damage, the formation of 8-hydroxydeoxyguanosine (8-OHdG) is a ubiquitous marker of oxidative stress. 8-OHdG, one of the oxidative DNA damage byproducts, is physiologically formed and enhanced by chemical carcinogens. During the repair of damaged DNA in vivo by exonucleases, the resulting 8-OH-dG is excreted without further metabolism into urine.
Cell Biolabs’ Oxidative DNA Damage ELISA Kit is a competitive enzyme immunoassay developed for rapid detection and quantitation of 8-OHdG in urine, serum, or other cell or tissue DNA samples. The quantity of 8-OHdG in unknown sample is determined by comparing its absorbance with that of a known 8-OHdG standard curve. The kit has an 8-OHdG detection sensitivity range of 100 pg/mL to 20 ng/mL. Each kit provides sufficient reagents to perform up to 96 assays, including standard curve and unknown samples.