FITC标记的去整合素样金属蛋白酶13抗体
产品名称： FITC标记的去整合素样金属蛋白酶13抗体
英文名称： Anti-ADAM13/FITC
产品编号： HZ-
产品价格： null
产品产地： 中国/上海
品牌商标： HZbscience
更新时间： 2023-08-17T10:24:20
使用范围： ICC=1:50-200 IF=1:50-200
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Rabbit Anti-ADAM13/FITC Conjugated antibody
FITC标记的去整合素样金属蛋白酶13抗体
| 英文名称 | Anti-ADAM13/FITC |
| 中文名称 | FITC标记的去整合素样金属蛋白酶13抗体 |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 研究领域 | 细胞生物 神经生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Xenopus (Silurana) tropicalis |
| 产品应用 | ICC=1:50-200 IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 68kDa |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from Xenopus (Silurana) tropicalis ADAM13 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: ADAM13 was first described as a protein expressed in somatic mesoderm and neural crest cells, in developing Xenopus embryos. ADAM13 was also found in liver, heart, and intestines from adult Xenopus. ADAM13 may regulate cellular signaling via Src and Src tyrosine kinase. ADAM13 may also act as a cell attachment molecule, by binding integrins through the cysteine rich domain amoung many other roles. A member of the metalloproteinase family containing disintegrin like domains (ADAMs) the functions of ADAM13 are still poorly understood. ADAM13 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, as well as disintegrin, cysteine rich, EFG like, transmembrane and Cytoplasmic domains. ADAM13 has been shown to be proteolytically active, cleaving fibronectin after binding it to the EGF like domain. ADAM13 is also shed from cells in culture, cleaved aminoterminal from the transmembrane domain, and is released into the culture media. Shed ADAM13 is a 52 kD protein, and can form complexes with a2 macroglobulin, suggesting it is a competent protease. Xenopus ADAM13 has greatest homology with human ADAM 33 (51% identical), and is 46% identical with human or mouse ADAM12 or ADAM19. It is still unclear if any of these ADAMs are species orthologs of Xenopus ADAM13, but there are significant differences between the related sequences, suggesting that ADAM13 may be a unique protein. The full length Xenopus ADAM13 sequence codes for a 914 amino acid protein. Predicted mass is 99.749 kD, but glycosylation and cyteine rich regions give Xenopus ADAM13 an apparent MW of 120 kD unprocessed, and 97 kD processed forms, on reduced SDS PAGE gels. ADAM13 contains a putative furin cleavage site, suggesting that a prohormone convertase cleaves the propeptide domain away from the catalytic domain Database links: Entrez Gene: 386623 Xenopus laevis Unigene: 166 Xenopus laevis Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications |
ADAM13首次被描述为在体细胞中胚层和神经嵴细胞中表达的蛋白，在开发爪蟾胚胎中。ADAM13也存在于爪蟾肝脏、心脏和肠中。ADAM13可通过Src和Src tyrosine kinase调节细胞信号转导。ADAM13也可以作为细胞附着分子，通过结合整合素通过富含半胱氨酸的域，起到许多其他作用。含有解整合素样结构域（ADAM）的金属蛋白酶家族的成员，对ADAM13的功能仍知之甚少。ADAM13含有典型的HExXHxxxxH锌金属蛋白酶基序，以及去整合素、富含半胱氨酸、EFG样跨膜结构域和细胞质结构域。ADAM13已被证明是蛋白水解活性的，在结合到EGF样结构域后裂解纤维连接蛋白。ADAM13也从培养细胞中脱落，从跨膜结构域裂解氨基甲酸酯，并释放到培养基中。脱落ADAM13是一个52 kD的蛋白质，可以与A2巨球蛋白形成复合物，表明它是一种有竞争力的蛋白酶。爪蟾ADAM13与人ADAM 33具有最大的同源性（51%相同），与人或小鼠ADAM12或ADAM19有46%的同源性。目前尚不清楚这些ADAM是否是爪蟾ADAM13的种类同源性，但相关序列之间存在显著差异，表明ADAM13可能是一种独特的蛋白质。全长爪蟾ADAM13序列编码914个氨基酸的蛋白质。预测的质量是99.749kD，但糖基化和富含细胞氨酸的区域使爪蟾ADAM13在还原的SDS PAGE凝胶上具有120kD未加工和97kD加工形式的表观MW。ADAM13含有一个推测的呋喃裂解位点，表明促性激素转换酶将前肽结构域从催化结构域中分离出来。