Lipofectin Transfection Reagent is the reagent of choice for transfection of endothelial cells. Lipofectin Transfection Reagent is also suitable for transfecting DNA, RNA, and oligonucleotides into mammalian cells, and DNA into plant protoplasts. Lipofectin reagent has also been shown to work well, in combination with PLUS Reagent, for the transfection of HeLa cells. Lipofectin Transfection Reagent is a 1:1 (w/w) liposome formulation of the cationic lipid N-[1-(2,3-dioleyloxy)propyl]-n,n,n-trimethylammonium chloride (DOTMA) and dioleoyl phophotidylethanolamine (DOPE) in membrane-filtered water.