IL-6 is a pleiotropic cytokine that plays an important role in host defense by regulating immune and
inflammatory responses. Produced by T cells, monocytes, fibroblasts, endothelial cells and keratinocytes, IL-6
has diverse biological functions. It stimulates B cell differentiation and antibody production, synergizes with IL-
3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins,
and regulates bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains, IL-6R
α and gp130. Murine IL-6 is inactive on human cells, while both human and murine are equally active on
murine cells.
The BioGems Murine IL-6 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase
immunoassay specially designed to measure Murine IL-6 with a 96-well strip plate that is pre-coated with rat
monoclonal antibody specific for IL-6. It contains E. coli-expressed recombinant Murine IL-6. The detection
antibody is a biotinylated goat polyclonal antibody specific for IL-6. The kit is analytically validated with ready
to use reagents.