MAP3K3 (Center) Peptide
产品名称： MAP3K3 (Center) Peptide
英文名称： MAP3K3 (Center) Peptide
产品编号： P2888
产品价格： 0
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围： null
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Rabbit polyclonal antibody raised against synthetic peptide of MAP3K3.
- Immunogen:
- A synthetic peptide (conjugated with KLH) corresponding to internal region of human MAP3K3.
- Host:
- Rabbit
- Reactivity:
- Human
- Form:
- Liquid
- Purification:
- Protein A purification
- Storage Buffer:
- In PBS (0.09% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Recommend Usage:
- ELISA (1:1000)
Western Blot (1:50-100)
Immunohistochemistry (1:50)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- Direct activation of the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by an inducible mitogen-activated protein Kinase/ERK kinase kinase 3 (MEKK) derivative.
Ellinger-Ziegelbauer H, Brown K, Kelly K, Siebenlist U.J Biol Chem. 1997 Jan 31;272(5):2668-74.
- Applications
- Western Blot (Cell lysate)
- Western blot analysis of MAP3K3 polyclonal antibody (Cat # PAB2275) in K-562 cell line lysates (35 ug/lane). MEKK3 (arrow) was detected using the purified polyclonal antibody (1 : 60 dilution).
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Formalin-fixed and paraffin-embedded human brain tissue reacted with MAP3K3 polyclonal antibody (Cat # PAB2275) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Entrez GeneID:
- 4215
- Protein Accession#:
- NP_976226;Q99759
- Gene Name:
- MAP3K3
- Gene Alias:
- MAPKKK3,MEKK3
- Gene Description:
- mitogen-activated protein kinase kinase kinase 3
- Omim ID:
- 602539
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene product is a 626-amino acid polypeptide that is 96.5% identical to mouse Mekk3. Its catalytic domain is closely related to those of several other kinases, including mouse Mekk2, tobacco NPK, and yeast Ste11. Northern blot analysis revealed a 4.6-kb transcript that appears to be ubiquitously expressed. This protein directly regulates the stress-activated protein kinase (SAPK) and extracellular signal-regulated protein kinase (ERK) pathways by activating SEK and MEK1/2 respectively; it does not regulate the p38 pathway. In cotransfection assays, it enhanced transcription from a nuclear factor kappa-B (NFKB)-dependent reporter gene, consistent with a role in the SAPK pathway. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq
- Other Designations:
- MAP/ERK kinase kinase 3,MAPK/ERK kinase kinase 3
- Related Disease