REST抗体-抗体-抗体-生物在线
上海信裕生物科技有限公司
REST抗体

REST抗体

商家询价

产品名称: REST抗体

英文名称: RESTAntibody

产品编号: XY22242-1

产品价格: null

产品产地: 中国/美国

品牌商标: XYbscience

更新时间: 2023-08-17T09:55:27

使用范围: WB,ELISA,IHC-P,IHC-F,IF

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 REST抗体Applications

In-house applications ELISA, WB, IP     Application not tested? Please see our Guarantee.
Published applications IF, WB Publications 1 citations –view here
Species specificity Human; other species not tested.
Published Species Rat
Images
Prev1NextView more validation data
  • 22242-1-AP;L02 cellsL02 cells were subjected to SDS PAGE followed by western blot with 22242-1-AP( REST Antibody) at dilution of 1:600
  • 22242-1-AP;HeLa cellsHeLa cells were subjected to SDS PAGE followed by western blot with 22242-1-AP( REST Antibody) at dilution of 1:1500
  • 22242-1-AP;HeLa cellsIP Result of anti-REST (IP:22242-1-AP, 5ug; Detection:22242-1-AP 1:1000) with HeLa cells lysate 2720ug.
Positive WB detected in L02 cells, HeLa cells, HepG2 cells
Positive IP detected in HeLa cells
Recommended dilution WB : 1:200-1:2000
IP : 1:500-1:5000
 REST抗体Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
Product Information
Source Rabbit Purification method Antigen affinity purification
Isotype IgG Storage PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC.
Immunogen Information REST抗体
Immunogen REST fusion protein ag17707 Full name RE1-silencing transcription factor
Calculated molecular
weight
1097aa,122 kDa Observed molecular 
weight
200 kDa
GenBank accession 
number
BC132859 Gene ID (NCBI) 5978
Gene symbol REST Synonyms NRSF, REST, X2 box repressor, XBR
Background

REST also named as NRSF or X2 box repressor is a 1097 amino acid protein, which contains 9 C2H2-type zinc fingers. REST as transcriptional repressor binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Repressor element 1 (RE1) silencing transcription factor (REST), is widely expressed during embryogenesis, and plays a strategic role in terminal neuronal differentiation. The molecular weight of REST is 121 kDa, but REST was detected 200kDa by western blot in our result, which is similar to the one article (PMID: 16764822). REST抗体

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