pBiFC-bFOSDelataZipVC155

·         Gene/insert name:

bFosDelataZIPVC155

·         Insert size:

551

·         Species:

R. norvegicus (rat)

·         Species:

Aequorea Victoria

·         Fusion protein or tag:

HA

·         Terminal:

N terminal on backbone

·         Mutation:

deletion of residues 179-193 in bFos(118-211)

·         Vector backbone:

pCMV-HA
(Search Vector Database)

·         Backbone manufacturer:

Clontech

·         Vector type:

Mammalian Expression

·         Backbone size w/o insert:

3756

·         Cloning site 5:

EcoRI

·         Site destroyed during cloning:

No

·         Cloning site 3:

KpnI

·         Site destroyed during cloning:

No

·         5 sequencing primer:

n/a List of Sequencing Primers

·         3 sequencing primer:

n/a

·         Bacterial resistance:

Ampicillin

·         Growth strain:

DH5alpha

·         Growth temperature (℃):

37

·         Growth instructions:

DH5 alpha

·         High or low copy:

High Copy

·         Sequence:

 >Author sequence

GAGTTCGAGCTTGCATGCCTGCAGGTCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCA

ACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTG

ACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGT

ACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGG

ACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTA

CATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGG

AGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAA

TGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCAGATCGCCTG

GAGACGCCATCCACGCTGTTTTGACCTCCATAGAAGACACCGGGACCGATCCAGCCTCCGGACTCTAGAG

GATCCGGTACTAGAGGAACTGAAAAACCAGAAAGTTAACTGGTAAGTTTAGTCTTTTTGTCTTTTATTTC

AGGTCCCGGATCCGGTGGTGGTGCAAATCAAAGAACTGCTCCTCAGTGGATGTTGCCTTTACTTCTAGGC

CTGTACGGAAGTGTTACTTCTGCTCTAAAAGCTGCGGAATTGTACCCGCGGGCCCACCATGTACCCATAC

GATGTTCCAGATTACGCTCTTATGGCCATGGAGGCCCGAATTCGGGGTCGTGCGCAGTCCATCGGTCGTC

GCGGTAAAGTTGAACAACTGTCCCCGGAAGAGGAAGAGAAACGTCGCATCCGCCGTGAACGTAACAAAAT

GGCGGCAGCGAAATGCCGTAACCGCCGTCGTGAACTGACCGACACCCTGCAGGCGGAAACCGACCAGCTG

GAAGACGAAAAATCCGCGGAGTTCATCCTGGCGGCACACCGTCCGGCGTGCAAAATCCCGAACGACCTGG

GTGGTACCCGTCCGGCGTGCAAAATCCCGAACGACCTGAAACAGAAAGTCATGAACCACGACAAGCAGAA

GAACGGCATCAAGGCCAACTTCAAGATCCGCCACAACATCGAGGACGGCGGCGTGCAGCTCGCCGACCAC

TACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCTACCAGT

CCAAACTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGG

GATCACTCTCGGCATGGACGAGCTGTACAAGTAAGCGGCCGCGGGGATCCAGACATGATAAGATACATTG

ATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTAT

TGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTTATGTTT

CAGGTTCAGGGGGAGGTGTGGGAGGTTTTTTCGGATCCTCTAGAGTCGATCTGCAGGCATGCTAGCTTGG

CGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGC

CGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCA

CTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAG

GCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCG

GCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAG

AACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATA

GGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACT

ATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACC

GGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCA

GTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGC

CTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACT

GGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACG

GCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGG

TAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACG

TUNEology 波长可调检测卡盒-->