产品名称:JC-1
别名:5,5',6,6'-Tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine iodide
产品描述:The membrane-permeant dual-emission potential-sensitive JC-1 dye is widely used in apoptosis studies to monitor mitochondrial health by flow cytometry, fluorescence microscopy and in microplate-based fluorescent assays. JC-1 dye can be used as an indicator of mitochondrial membrane potential in a variety of cell types, including myocytes and neurons, as well as in intact tissues and isolated mitochondria. JC-1 accumulates in mitochondria, selectively generating an orange J-aggregate emission profile (590 nm) in healthy cells. After cell injury, as membrane potential decreases, JC-1 monomers are generated, resulting in a shift to green emission (529 nm). The principal advantage of JC-1 relative to other commonly employed fluorescent probes of mitochondrial membrane potential is that it allows qualitative visualization, considering the shift from orange to green fluorescence emission, and quantitative detection, considering the fluorescence intensity ratio.
保存温度:-20°C
运输温度:Ambient
预期分子量:652.2
产品形式:liquid
有效期:Stable for at least 2 years after receipt when stored at -20°C.
产地:瑞士
生产商:AdipoGen Life Sciences
CAS号:3520-43-2 and 47729-63-5
分子式:C25H27Cl4IN4
溶解度:Soluble in DMSO, ethanol, methanol or acetone.
文献引用：
1.J-aggregate formation of a carbocyanine as a quantitative fluorescent indicator of membrane potential: M. Reers, et al.; Biochemistry 30, 4480 (1991)
2.Intracellular heterogeneity in mitochondrial membrane potentials revealed by a J-aggregate-forming lipophilic cation JC-1: S.T. Smiley, et al.; PNAS 88, 3671 (1991)
3.A new method for the cytofluorimetric analysis of mitochondrial membrane potential using the J-aggregate forming lipophilic cation 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolcarbocyanine iodide (JC-1): A. Cossarizza, et al.; BBRC 197, 40 (1993)
4.Mitochondrial membrane potential monitored by JC-1 dye: M. Reers, et al.; Methods Enzymol. 260, 406 (1995)
5.JC-1, but not DiOC6(3) or rhodamine 123, is a reliable fluorescent probe to assess delta psi changes in intact cells: implications for studies on mitochondrial functionality during apoptosis: S. Salvioli, et al.; FEBS Lett. 411, 77 (1997)
订购详情：
| 品牌 | 产品名称 | 产品货号 | 规格 |
| Adipogen | JC-1 |
AG-CR1-3568-M001 AG-CR1-3568-M005 AG-CR1-3568-5001 |
1mg 5mg 5 x 1 mg |
详情请咨询Adipogen全国授权一级代理-欣博盛生物科技
全国服务热线: 4006-800-892 邮箱: market@neobioscience.com
深圳: 0755-26755892 北京: 010-88594029 上海: 021-34613729
广州：020-87615159 香港: 852-69410778
代理品牌网站: www.neobioscience.com
自主品牌网站: www.neobioscience.net